ror1-pe  (R&D Systems)

Journal: Cell Reports Methods

Article Title: Sequence enrichment profiles enable target-agnostic antibody generation for a broad range of antigens

doi: 10.1016/j.crmeth.2023.100475

Figure Lengend Snippet:

Article Snippet: PE Mouse anti-Human ROR1 , BD Biosciences , Cat# 564474; RRID: AB_2738822.

Techniques: Produced, Recombinant, Sequencing, Software

Journal: Leukemia research

Article Title: ROR1 targeted immunoliposomal delivery of OSU-2S shows selective cytotoxicity in t(1;19)(q23; p13) translocated B-cell acute lymphoblastic leukemia

doi: 10.1016/j.leukres.2022.106872

Figure Lengend Snippet: A) ROR1 Mean Fluorescence Intensity (MFI) normalized to isotype (ΔMFI) of non t(1;19) and t(1;19) translocated B-ALL samples. Translocated patient derived cells show specific ROR1 expression (n = 3, p = 0.0002, mean difference in MFI 403.3 ± 48.04). Histograms show representative surface ROR1 expression in t(1:19) translocated and non t(1:19) B-ALL patient derived CD19 cells. B) ROR1 Mean Fluorescence Intensity (MFI) normalized to isotype (ΔMFI) of non t(1;19) (NALM-6, RS4;11) and t(1;19) translocated (697) B-ALL cell lines. t(1;19) translocated cells show specific ROR1 expression (n = 3, p = <0.0001, mean increase in MFI 697 vs NALM-6 = 2528, 697 vs RS4;11 = 2591). C) Representative histograms showing ROR1 expression on 697 cells but not non t(1;19) NALM-6 and RS4;11 cells as detected by flow cytometry. D) Scheme depicting preparation and expected action of ROR1 targeting immunoliposomes encapsulating OSU-2S (2A2-OSU-2S-ILP). E) Representative analysis of size and concentration of 2A2-OSU-2S-ILPs using Nanoparticle Tracking Analysis by NanoSight. Mean size was 186.9 +/− 0.8nm, mean concentration was 1.2×1013 +/− 1.05 × 1011 particles/ml. F) 2A2-OSU-2S-ILPs mediate selective cytotoxicity in ROR1 + 697 cells as compared to IgG-OSU-2S-ILP (n = 8, p < 0.0001, mean decrease in viability 61.62%). G) 2A2-OSU-2S-ILPs mediate selective cytotoxicity in ROR1 + t(1:19) primary ALL cells as compared to IgG-OSU-2S-ILP and Empty ILPs (n = 3, p = 0.0174, mean decrease in viability 35.14%). H) No significant relative cytotoxicity was observed with 2A2-OSU-2S-ILPs in ROR1- non t(1;19) cell line (NALM-6) and B-ALL primary cells. I) 2A2-OSU-2S-ILP significantly reduced tumor burden in the bone marrow of treated mice as compared to IgG control as detected by human CD45+/CD19+cells [p = 0.022, n = 5 (IgG-OSU-2S-ILP), n = 6 (2A2-OSU-2S-ILP), mean decrease = 1.751 ± 0.6372 × 106 cells. J) 2A2-OSU-2S-ILP (n = 9) treatment significantly improves survival in 697 CDX model as compared to (n = 6) IgG-OSU-2S-ILP (p = 0.013) and (n = 5) 2A2-Empty-ILP (p = 0.0044) treated mice.

Article Snippet: Antibodies used include c-Myc, p21 and β-Actin (Cell Signaling, Danvers, MA), CD45-FITC, CD3-PE-Cy7, CD19-BV785 (BioLegend, San Diego, CA), murine CD45-PECF594 (BD Biosciences) and ROR1- PE (R&D Systems).

Techniques: Fluorescence, Derivative Assay, Expressing, Flow Cytometry, Concentration Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Role of casein kinase 1 in the amoeboid migration of B-cell leukemic and lymphoma cells: A quantitative live imaging in the confined environment

doi: 10.3389/fcell.2022.911966

Figure Lengend Snippet: Expression of surface markers (A) CCR7 (CCL19 receptor), CD29 and CD49d (VLA4 integrin subunits), (B) ROR1 and CXCR4 (CXCL12 receptor), and CD11a and CD18 (LFA1 integrin subunits) in HG-3, MAVER-1, MEC-1, and MINO cells. Stained (red) and unstained control (blue) samples are shown as dot plots of fluorescence intensity. Three replicate measurements for each cell line are shown; for quantification, see . (C) Representative tSNE visualization of metaclusters automatically generated by FlowSOM of HG-3, MAVER-1, MEC-1, and MINO cells from one replicate shown in (A–B) . (D) tSNE visualization of cell line distribution. (E) Left: tSNE of the HG-3 cell line upon subtraction from the dataset shown in 5C. Right: heatmap resuming expression of individual markers in subclusters of the HG-3 cell line. (F) Left: tSNE of the MINO cell line upon subtraction from the dataset shown in 5C. Right: heatmap resuming expression of individual markers in subclusters of the MINO cell line.

Article Snippet: HG-3 cells were stained using the ROR1-PE antibody (cat. no. 130-098-317, Miltenyi Biotec) using the same staining protocol as described earlier.

Techniques: Expressing, Staining, Fluorescence, Generated